GreenHab Report – April 17th

Crew 280 GreenHub Report 17-04-2023

GreenHab Officer: Laia Ribas

Environmental control: Open during day and heater during night

Average temperatures: 78.8 F – 87.7 F

Hours of supplemental light: N/A

Daily water usage for crops: 13 gallons

Daily water usage for research and/or other purposes: 0.5 gallons for fish purposes

Water in Blue Tank: 183 gallons (crops and fish)

Time(s) of watering for crops: 09:30, 20:00

Changes to crops: seed tomatoes are cultured

General watering of plants and crops was done today. The sun was very intense today, in particular at noon time. The door was left open all day and closed in the evening. The plants have been watered again in the evening at 20.00..

9 adult zebrafish (Danio rerio; 5 wild and 4 transgenics) adults were obtained from a pet store in Grand Junction (Colorado, USA) J&M Aquatics & Pet Center. Fish were transported to MDRS and reared in a tank (30 L) with Hub water at SOL 0. Fish were acclimated by mixing the water. Fish were not fed. The fish were acclimated from SOL0 to SOL1 by periodically increasing the amount of water and adding the water filter to reduce the stress. In order to reduce the water used, less than half of the tank was filled. The water filter was set at 10.30 and the fish were fed. All animals ate except one transgenic which did not look healthy. For ethical reasons, this animal was sacrificed by cold shock for 10 min. At 17.00 all fish were found dead due to the high temperature in the tank. The tank water exceeds 98.6F. Fish were kept in the freezer.

Harvest: 1 g of two branches of thyme and eight leaves of sage, 114 g microgreens, 56 g cherry tomatoes

Support/supplies needed:
We request help to provide new zebrafish (see SOL1 summary).

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